Globin protein expression by ES-derived erythroid cells. Expression of ϵ, ζ, γ, and β/δ globin proteins was detected in the colony-derived erythroid cells from D14EBs, from nonadherent cells, or from expanded nonadherent cells by staining with specific globin antibodies followed by an FITC-conjugated anti-mouse antibody and counterstained with DAPI (4′6-diamidino-2-phenylindole 2HCl). Pictures were taken under a Leica DMLB microscope with a 40 ×/0.70 NA PL Fluotar objective lens using an RT Slider Spot camera (Diagnostic Instruments, Sterling Heights, MI) with spot software version 4.0.9.