Histology. Hematoxylin-eosin (A,C), silver stain (B,D), and trichrome (E) colorations of the marrow (A-B) and spleen (C-E) are shown. Control (JAK2^WT) and JAK2^V617F-recipient mice with posttransplantation time (< 3 months or > 4 months) are indicated with main features: trilineage hyperplasia in early stages and fibrosis/low cellularity in late stages. Images were obtained using a Leica DMRB microscope (Leica, Solms, Germany) with lenses ×20 (0.5 numeric aperture) × 10 = 200 × total magnification, and acquired with a Video 3 charge-coupled device (CCD) Sony Leica Power hole accumulated diode (HAD) camera (Sony, Tokyo, Japan).