VEGF₁₆₅-induced proliferation of MMECs (with or without FCS 10%) in the presence of different SEMA3A doses, or anti-NRP1 and/or anti-VEGFR-2 antibodies. Data are presented as mean plus or minus 1 SD (A). Effect of VEGF₁₆₅, alone or in the presence of SEMA3A, on MMEC chemotaxis. Bars represent the mean plus or minus 1 SD of the number of migrated cells per 5 high power fields in triplicate wells (B). Significance of changes by the Wilcoxon-Wilcox test. Gene modulation in presence of exogenous SEMA3A by RT-PCR and real-time RT-PCR (C) at different times of collection. Data are expressed as fold expression of baseline (time 0) value.