Four hours of treatment with SAHA and ITF2357 are required to prevent IL-1β secretion. Monocytes were stimulated with LPS and treated without or with 50 nM ITF2357 or 0.5 μM SAHA for the entire time of culture (4h) or left untreated the first 2 hours and treated the last 2 hours (2h), or left untreated 3 hours and treated the last 1 hour (1h). At the end of the incubation time, cells were triggered for 10 minutes by ATP and secretion of IL-1β, caspase-1, and cathepsin D was evaluated by Western blot followed by densitometric analyses. Data are expressed as percent of inhibition of secretion of IL-1β, caspase-1, and cathepsin D. One representative experiment of 4 performed is shown.