American Society of Hematology

Figure 4.

$Figure 4. Inhibition of the PI3K/Akt pathway decreases activation of the NF-κB pathway. PI3K/Akt pathway inhibition studies were performed with the Granta 519 and Z138C cell lines using 3 different inhibitors: LY294002, Akt inhibitor, and wortmannin. Comparable results were obtained for both lines, and results with Granta 519 using LY294002 or Akt inhibitor are shown as representative experiments. (A,C) Western blot analysis reveals Akt inactivation by 8 hours, and a time-dependent decrease in phosphorylated IκBα and p65. Total Akt and α-tubulin are shown as loading controls. (B,D) Western blot analysis of separated nuclear and cytoplasmic fractions reveals a gradual accumulation of the NF-κB p65 subunit in the cytoplasm following addition of LY294002 or Akt inhibitor and a corresponding reduction in nuclear NF-κB. α-tubulin is shown as loading control for cytoplasmic protein; oct-1 as loading control for nuclear protein.$

Inhibition of the PI3K/Akt pathway decreases activation of the NF-κB pathway. PI3K/Akt pathway inhibition studies were performed with the Granta 519 and Z138C cell lines using 3 different inhibitors: LY294002, Akt inhibitor, and wortmannin. Comparable results were obtained for both lines, and results with Granta 519 using LY294002 or Akt inhibitor are shown as representative experiments. (A,C) Western blot analysis reveals Akt inactivation by 8 hours, and a time-dependent decrease in phosphorylated IκBα and p65. Total Akt and α-tubulin are shown as loading controls. (B,D) Western blot analysis of separated nuclear and cytoplasmic fractions reveals a gradual accumulation of the NF-κB p65 subunit in the cytoplasm following addition of LY294002 or Akt inhibitor and a corresponding reduction in nuclear NF-κB. α-tubulin is shown as loading control for cytoplasmic protein; oct-1 as loading control for nuclear protein.

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