Figure 1.
Arginase is liberated from dying human PMNs. Purified human PMNs (5 × 106 cells per 200 μL in PBS) were cultured for the indicated time. Cellfree supernatant was harvested after centrifugation, and protein concentration as well as arginase activity were determined in the supernatant. Arginase activity is shown as milliunits per milligram of protein (A) and milliunits per milliliter of supernatant (B). Data are representative of 3 different experiments (mean of triplicates ± SD).
