Alde-Low EPCs were curative of ischemic tissue. (A) Skin incisions were created on the dorsal surfaces of mice, and PBS alone, HUVECs, Alde-High EPCs, or Alde-Low EPCs were injected (top and bottom photographs/each condition). The effectiveness of ECs to recover tissue from ischemia was examined on day 7 following surgery. (B) The necrotic regions in the 4 types of mice (n = 3) were measured. Note that the area of necrosis was smallest in the mice injected with Alde-Low EPCs. Gray bar indicates PBS; striped bar, HUVECs; black bars Alde-High EPCs; and white bar, Alde-Low EPCs. **P < .01. (C) Neovascularization was examined in the region of the incision after the injection of Alde-Low EPCs. Newly formed vessels were analyzed for the detection of GFP-positive Alde-Low EPCs (GFP; top) and whole vessel formation by TRIC-conjugated lectin (GFP-TRIC-Lectin; bottom). The colocalization of GFP-positive EPCs and TRIC-positive fine capillaries can be observed (GFP-TRIC-Lectin, arrowheads). The numbers of GFP-positive HUVECs, Alde-High EPCs, and Alde-Low EPCs that had migrated to the dorsal skin were scored on the seventh day after surgery (left). *P < .05. Bar indicates 100 μm.