Functional p53 is not required for chloroquine or 3-methyladenine to synergize with SAHA. (A) shRNA-mediated knockdown of p53 in Ba/F3 p210 and Ba/F3 T315I cells. Actin was used as a loading control. (B) Quantification of drug-induced apoptosis. Vector control and p53 shRNA-expressing p210 and T315I Ba/F3 cells were treated with 25 μM CQ, 2 mM 3-MA, 1 μM SAHA, or the indicated combinations for 48 hours. Percentages of apoptotic cells were quantified by PI/FACS. Bars represent the mean of 3 independent experiments. Error bars indicate the SEM. *P < .05.