Transfer of the thymus disorder by Nanog T cells. Nanog T cells were generated in a recipient that was irradiated and reconstituted by Nanog KSL cells. Eight weeks later, eGFP⁺ cells were purified from the thymus, and 10⁵ cells were transferred to untreated secondary recipients. As a control, the same number of eGFP⁺ cells generated in the thymus of recipients reconstituted by KSL transduced with the control vector was transferred. The surface phenotype of eGFP⁺ cells in the primary recipients is shown in the upper panels. The chimerism of eGFP⁺ cells in secondary recipients is shown in the middle panels, and total cellularity of thymus is also shown. The surface phenotype of eGFP⁺ and eGFP⁻ cells in the thymus of the secondary recipients were also analyzed (bottom panels).