Figure 3
![Figure 3. CD40-mediated B-cell proliferation and up-regulation of surface markers. (A) Proliferation was measured in splenic B cells treated with IL-4 either with or without hCD40L for 3 days. Cultures were pulsed with [3H]TdR for the last 8 hours of culture (*P = .005; **P = .008). (B) For the induction of surface markers, splenic B cells were cultured in vitro for 48 hours with or without hCD40L (400 ng/mL). Cells were harvested and stained for CD23 and CD80 followed by FACS analysis. Data are representative of more than 3 independent experiments as the average ± standard deviation (SD).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/110/1/10.1182_blood-2006-07-038414/2/zh80130702820003.jpeg?Expires=1736047393&Signature=19qV0HYTNtz6t9kW1MOxFrb~-pRurhvqRbSEuJBDeUaq7ORuDQD7M0r0EaWvMYHk3khiKMUJtONaYn~rftPk8Xx6kLvRkwyaWE-HGwfL8hJKfLhpkq7P79sms1mjF8y-YvZRRzq50J--r3Uy-9EhuDHVAW1EoszNYQ4n5hX~aXL4nPSBhK6i5xa-roksiJ7cfPhy8kMduOZqlC-T4otQB0ZdDGdXIjFql7eQ4l2yBZFNEiTCWYn43XolWKmhx4a832SJaxWjzzcKEbCBflIadyyWnq1pWuzhDt0xywwlGSvb~WjQitPpzsO5i2qIuNB1yBzKdfUlEHVwqEX94uGnBw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD40-mediated B-cell proliferation and up-regulation of surface markers. (A) Proliferation was measured in splenic B cells treated with IL-4 either with or without hCD40L for 3 days. Cultures were pulsed with [3H]TdR for the last 8 hours of culture (*P = .005; **P = .008). (B) For the induction of surface markers, splenic B cells were cultured in vitro for 48 hours with or without hCD40L (400 ng/mL). Cells were harvested and stained for CD23 and CD80 followed by FACS analysis. Data are representative of more than 3 independent experiments as the average ± standard deviation (SD).
