Effect of PAR agonists on intracellular tissue factor mobilization. Fibroblasts were exposed to control vehicle, FVIIa (10 nM), FFR-FVIIa (10 nM), PAR agonist peptides (50 μM), thrombin (10 nM), or trypsin (10 nM) for 2 hours at 37°C. Cells were fixed, permeabilized, and immunostained with rabbit polyclonal antihuman TF and monoclonal antihuman golgin-97 antibodies, followed by Rhodamine Red–labeled antirabbit and Oregon Green–labeled antimouse antibodies as secondary reporter antibodies. Left panel images represent TF staining, middle panel images represent the Golgi staining, and right panel images represent the overlay of TF and golgin-97 staining (colocalization). Inserts show a magnified view of TF localization in the Golgi.