Inhibition of endothelial cell migration. CXCL11(1-73), CXCL11(3-73), and CXCL11(7-73) were tested for their ability to inhibit endothelial cell migration in vitro. Scars were drawn in a confluent HMVEC monolayer and the alteration in width upon addition of chemokine was scored microscopically after 24 hours. Zero scores correspond to the change in width for untreated control cultures. A scar that retained a broader width compared with control scars (inhibition of migration) received a negative inhibition score. Panels A, B, C, and D are representative examples of inhibition of migration with width scores 0, −1, −2.25, and −2.5, respectively. Results in panel E represent average changes in scores (± SEM) for 8 to 10 experiments, each tested in duplicate wells in one 24-well plate. Each well was scored double blind by 2 investigators. Statistical analysis of inhibition of HMVEC migration between different CXCL11 forms was performed with the Mann-Whitney U test [*P < .05, **P < .01, and ***P < .001 for CXCL11 isoforms compared with buffer-treated controls; ++P < .01 for CXCL11(1-77) compared with CXCL11(7-77); and X for P < .05 for CXCL11(3-73) compared with CXCL11(7-73)].