Myeloid dendritic cells in the spleen and inguinal lymph nodes from MRL/lpr mice. (A) The CD11c⁺ DC cell number of the spleen and ILNs is shown as means ± SD of 5 to 7 MRL/lpr and control mice at 4 and 12 weeks of age.(B) Mononuclear cells were isolated from the spleen and ILNs at 12 weeks of age, and the proportions were determined using flow cytometry. Shown are representative plots of CD8α⁺, CD11b⁺, B220⁺, and CD11c⁺ cells. Percentages in each region indicate the frequency of lymphoid (CD11c⁺CD11b⁻CD8α⁺), myeloid (CD11c⁺CD11b⁺CD8α⁻), and plasmacytoid (CD11c⁺B220⁺) DCs. Data are representative of 5 to 7 mice in each group. (C) Graph shows the mean frequency of lymphoid, myeloid, and plasmacytoid DCs. *P > .05; **P > .01 MRL/lpr versus MRL^+/+ mice. Results are representative of 3 independent experiments.