Reconstitution of T cells in lymph nodes after transplantation. (A) Frequencies of CD3⁺ T cells in lymph nodes before and after transplantation; animals given multiple doses of KGF are indicated with blue symbols, those given a single dose of KGF with green symbols, and control animals with red symbols. (B) and (C) Flow cytometry plots show T cell phenotyping in a representative animal before and 1 month after transplantation. (D) and (E) Frequencies of naive CD4⁺ and CD8⁺ T cells in lymph nodes obtained from the animals over time. Cells were gated firstly on small lymphocytes using scatter parameters, then on CD3⁺ T cells, and then subsets were analyzed within CD4⁺ or CD8⁺ populations using FlowJo software (TreeStar). Data are displayed are as median and interquartile range (IQR). For the 2 animals receiving multiple doses, only median is plotted as an IQR would be statistically unreliable. Significant P indicated for comparisons between all KGF-treated animals and controls.