Induction of Tregs by GILZ-expressing DCs. (A-C) DCs were nucleofected with siC or siGILZ and treated or not treated with DEX (10⁻⁷ M). (D-F) DCs were nucleofected with the control vector (pCTRL, ▩) or the GILZ-encoding vector (pGILZ, −). (A,D) Production of GILZ was analyzed by immunoprecipitation. Quantification of actin was used as an internal control. (B-C,E-F) DCs were pulsed with PPD and used to stimulate autologous CD4⁺ T lymphocytes. Expression of Treg markers was determined by flow cytometry. Staining with a control mAb is shown as a dotted line in panels C and F. All results are representative of 3 to 5 experiments. MW indicates molecular weight markers.