American Society of Hematology

Figure 1

$Figure 1. Cytoplasmic HES-DFO does not block 59Fe2-Tf incorporation into heme. Reticulocytes were exposed to transient lysis and resealing, as described in Experimental Procedures. (A) Cells were resealed in the presence of FITC-dextran (green), treated with MitoTracker CMXRos (red), and evaluated by confocal microscopy. (B) Cells were resealed in the absence or presence of 1 mM HES-DFO and then treated with either 1 μM 59Fe-SIH2 or 1 μM 59Fe2-Tf for 2.5 hour at 37°C. Heme and nonheme fractions were separated, measured by gamma counting, and the percentage of cell-associated 59Fe in heme was plotted. Error bars represent standard deviations (n = 3).$

Cytoplasmic HES-DFO does not block ⁵⁹Fe₂-Tf incorporation into heme. Reticulocytes were exposed to transient lysis and resealing, as described in Experimental Procedures. (A) Cells were resealed in the presence of FITC-dextran (green), treated with MitoTracker CMXRos (red), and evaluated by confocal microscopy. (B) Cells were resealed in the absence or presence of 1 mM HES-DFO and then treated with either 1 μM ⁵⁹Fe-SIH₂ or 1 μM ⁵⁹Fe₂-Tf for 2.5 hour at 37°C. Heme and nonheme fractions were separated, measured by gamma counting, and the percentage of cell-associated ⁵⁹Fe in heme was plotted. Error bars represent standard deviations (n = 3).

This Feature Is Available To Subscribers Only