American Society of Hematology

Figure 3

$Figure 3. Utilization of 59Fe from Tf is more efficient than from 59Fe-ascorbate. (A) Concentration dependence: Cells were incubated for 30 minutes with the indicated concentrations of either 59Fe2-Tf or 59Fe(II)-ascorbate and the heme and nonheme fractions measured and displayed as pmol Fe per million cells. (B) Time dependence: Cells were incubated in 2 μM 59Fe2-Tf or 59Fe(II)-ascorbate for the indicated times and analyzed as in (A). Error bars represent standard deviations (n = 3).$

Utilization of ⁵⁹Fe from Tf is more efficient than from ⁵⁹Fe-ascorbate. (A) Concentration dependence: Cells were incubated for 30 minutes with the indicated concentrations of either ⁵⁹Fe₂-Tf or ⁵⁹Fe(II)-ascorbate and the heme and nonheme fractions measured and displayed as pmol Fe per million cells. (B) Time dependence: Cells were incubated in 2 μM ⁵⁹Fe₂-Tf or ⁵⁹Fe(II)-ascorbate for the indicated times and analyzed as in (A). Error bars represent standard deviations (n = 3).

This Feature Is Available To Subscribers Only