Trichimerism in cells derived from hematopoietic cells from peripheral blood, bone marrow, and lymph node. (A) Examples of marrow trichimerism in 3 of the dogs given marrow transplants from 2 DLA-identical donors determined at the weeks after HCT shown. (B) Chimerism analysis for dog G643, 34 weeks after HCT, of granulocytes and mononuclear cells from peripheral blood cells; CD45+ (leukocytes), CD34+ (progenitor stem cells), and DM5+ (myeloid cells) from bone marrow; CD3+ (T-cell antigen) and CD21 + (B-cell antigen) from lymph node cells. Cells were labeled with antibodies indicated above and sorted by FACS before determining chimerism.