American Society of Hematology

Figure 3

$Figure 3. Nuclear beta-catenin detection in UCB-CD34+ progenitors exposed to 2% to 3% O2 on PLL- versus FN-coated plates in the presence of osteoblast-derived cytokines and chemokine. CD34+ progenitors were cultured in SCF, IL-6, and CXCL12 for 48 hours on FN-coated plates in 2% to 3% versus 20% O2. Cells were normalized by cell count with Trypan blue to exclude dead cells. An average of 2 to 3 million cultured cells was subfractionated for protein analyses in the presence of NEM or ubiquitin aldehyde enzyme, to preserve the ubiquitinated form of beta-catenin. Tubulin and laminin were used as cytoplasmic and nuclear control, respectively.$

Nuclear beta-catenin detection in UCB-CD34⁺ progenitors exposed to 2% to 3% O₂ on PLL- versus FN-coated plates in the presence of osteoblast-derived cytokines and chemokine. CD34⁺ progenitors were cultured in SCF, IL-6, and CXCL12 for 48 hours on FN-coated plates in 2% to 3% versus 20% O₂. Cells were normalized by cell count with Trypan blue to exclude dead cells. An average of 2 to 3 million cultured cells was subfractionated for protein analyses in the presence of NEM or ubiquitin aldehyde enzyme, to preserve the ubiquitinated form of beta-catenin. Tubulin and laminin were used as cytoplasmic and nuclear control, respectively.

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