Bradykinin induces filopodia formation in endothelial cells. HUVECs were plated in serum-free medium containing growth factor supplement followed by the addition of bradykinin (15 minutes; 200 nM). Cells were fixed and stained with filipin; images were captured with a Zeiss Axioplan 2 (Thornwood, NJ) fluorescence microscope with 63×/1.4 NA oil objective, a Zeiss Axiocam HRC digital camera with plug-in v. 2.0 acquisition software, and processed with Photoshop 1 (Adobe, San Jose, CA). Inset shows immunofluorescence analysis of the filopodia marker fascin.