Figure 1
Figure 1. Detection of Fli-3 locus and transcripts in cell lines that do not express Fli-1. (A) High-molecular-weight DNA from cell lines KH9-ED, KH9-EI, KH11, KH14, and KH16, and control BALB/C mouse spleen cells was digested with EcoR1 and analyzed by Southern blot using the Fli-3 probe. The arrow shows the position of the rearranged bands. Hybridization with a Fli-1–specific probe was used to show equal loading. The intensity of KH9-ED band was used as control (1.0). The intensity of other bands was compared with the control. (B) Expression of Fli-3 in erythroleukemia cell lines. RNA isolated from the indicated cell lines was hybridized with the Fli-3 probe. Hybridization of the same blot with a GAPDH probe was used to show equal loading. The intensity of KH11 band was used as control (1.0). (C) Expression of Fli-3 in various murine tissues. Total RNA (20 μg) was isolated from various tissues and subjected to Northern blot analysis using the Fli-3 probe. (D) Expression of Fli-3 in various cell lines. Total RNA isolated from fibroblasts (NIH-3T3), erythroleukemia cell lines (DP18–9, CB3, CB7), breast cancer cell line (EMT6), a B-lymphocytic leukemia cell line (B10), a thymoma cell line (Yab-3), and endothelial cell line (SVR) was hybridized to the Fli-3 probe. The 18S rRNA from the ethidium bromide–stained gel was used to show equal loading. The intensity of CB3 band was used as control (1.0). (E) Expression of Fli-3 in murine hematopoietic cell lineages. E indicates erythroid cells; Meg, megakaryocyte; Mac, macrophage; Neu, neutrophil; BFU-E, burst forming units–erythroid; CFU-E, colony forming units–erythroid; Mast, mast cells; B, B lymphocyte; T, T lymphocyte; and p, unipotent precursor cells of 7-day colonies. The left column of each blot contains pentapotent (E/Meg/Mac/Neu/Mast), tetrapotent, tripotent, bipotent, and unipotent precursor cell cDNAs. Middle and right columns contain unipotent precursor cell cDNAs (pMac, pNeu, pMeg) and samples from terminally maturing cells (E, Neu, Mast, Meg, T, B). S17 and 95/1.7 are cDNA isolated from the bone marrow–derived fibroblastic cell lines. Quantification of radioactive signal intensity is shown at the bottom. Fold changes shown are mean values derived from 3 experiments.

Detection of Fli-3 locus and transcripts in cell lines that do not express Fli-1. (A) High-molecular-weight DNA from cell lines KH9-ED, KH9-EI, KH11, KH14, and KH16, and control BALB/C mouse spleen cells was digested with EcoR1 and analyzed by Southern blot using the Fli-3 probe. The arrow shows the position of the rearranged bands. Hybridization with a Fli-1–specific probe was used to show equal loading. The intensity of KH9-ED band was used as control (1.0). The intensity of other bands was compared with the control. (B) Expression of Fli-3 in erythroleukemia cell lines. RNA isolated from the indicated cell lines was hybridized with the Fli-3 probe. Hybridization of the same blot with a GAPDH probe was used to show equal loading. The intensity of KH11 band was used as control (1.0). (C) Expression of Fli-3 in various murine tissues. Total RNA (20 μg) was isolated from various tissues and subjected to Northern blot analysis using the Fli-3 probe. (D) Expression of Fli-3 in various cell lines. Total RNA isolated from fibroblasts (NIH-3T3), erythroleukemia cell lines (DP18–9, CB3, CB7), breast cancer cell line (EMT6), a B-lymphocytic leukemia cell line (B10), a thymoma cell line (Yab-3), and endothelial cell line (SVR) was hybridized to the Fli-3 probe. The 18S rRNA from the ethidium bromide–stained gel was used to show equal loading. The intensity of CB3 band was used as control (1.0). (E) Expression of Fli-3 in murine hematopoietic cell lineages. E indicates erythroid cells; Meg, megakaryocyte; Mac, macrophage; Neu, neutrophil; BFU-E, burst forming units–erythroid; CFU-E, colony forming units–erythroid; Mast, mast cells; B, B lymphocyte; T, T lymphocyte; and p, unipotent precursor cells of 7-day colonies. The left column of each blot contains pentapotent (E/Meg/Mac/Neu/Mast), tetrapotent, tripotent, bipotent, and unipotent precursor cell cDNAs. Middle and right columns contain unipotent precursor cell cDNAs (pMac, pNeu, pMeg) and samples from terminally maturing cells (E, Neu, Mast, Meg, T, B). S17 and 95/1.7 are cDNA isolated from the bone marrow–derived fibroblastic cell lines. Quantification of radioactive signal intensity is shown at the bottom. Fold changes shown are mean values derived from 3 experiments.

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