Sbds RNAi impairs in vitro granulocytic cell differentiation. Murine bone marrow cells were transduced with the indicated lentivirus, sorted for YFP expression, and either plated in suspension cultures containing SCF and G-CSF (A, C) or methylcellulose (B). (A) The number of viable cells was measured on initiation of the culture (day 0) and at the indicated times (representative of 3 independent experiments). (B) The number of CFU-Cs and CFU-Gs per 1000 c-Kit⁺, lineage⁻ cells is shown (n = 3-5). (C) After 7 days in vitro granulocytic differentiation, cells were resorted based on YFP expression, and leukocyte differentials were performed (n = 2-4). *P ≤ .05, ***P ≤.005. All data represent the mean (± SD).