Reduction in Sbds impairs short-term hematopoietic engraftment. (A) Lineage-depleted bone marrow cells were transduced with lentivirus, and transduction efficiency was assessed at least 48 hours later by flow cytometry. c-Kit⁺, lineage⁻ cells (box) were analyzed for YFP expression (representative of 5 independent high MOI transductions). (B) Kaplan-Meier survival curve of transplant recipients receiving donor bone marrow transduced with a high MOI (20:1). (C) Engraftment at 3 weeks in mice that received a transplant with cells transduced at an MOI of 10:1. Shown is the ratio of YFP percentage in the peripheral blood of engrafted recipients to the pretransplant transduction efficiency, as measured in c-Kit⁺, lineage⁻ donor bone marrow cells (n = 9-11; average ± SEM). *P ≤ .05, ***P ≤ .005.