Reduction in Sbds impairs HPC homing to the bone marrow. Bone marrow cells were transduced with the indicated lentivirus and transplanted into irradiated mice. (A) Bone marrow cells before transplantation and harvested from recipient mice 24 hours after transplantation were plated in methylcellulose, and the percentage of CFU-Cs that expressed YFP was determined. (B) Homing efficiency was measured as the ratio of after transplantation to before transplantation YFP⁺ CFU-Cs (n = 7-8). (C) A transwell migration assay was performed on transduced lineage⁻ bone marrow cells. Shown is the percentage of YFP⁺ and YFP⁻ CFU-Cs that migrated in response to CXCL12α (n = 3). *P ≤ .05, **P ≤ .01. All data represent the mean plus or minus SEM.