Long-term hematopoietic reconstitution by Sbds RNAi-transduced cells. (A) YFP expression in peripheral blood cells was measured at the indicated time after transplantation (n = 3-11). (B) Sbds mRNA relative to β-actin mRNA expression in mice 3 months after transplantation was measured by real-time RT-PCR (n = 2). The contribution of YFP⁺ granulocytes and B lymphocytes to the peripheral blood at 3 months (C) and the bone marrow at 6 months (D) was measured. Data were normalized to the pretransplant transduction efficiency. (E) The ability of YFP⁺ bone marrow cells to generate CFU-Cs and pre-B CFUs (6 months after transplantation), and CFU-GM, CFU-G, and BFU-E colonies (3 months after transplantation) was measured (n = 3-5). *P ≤ .05, **P ≤ .01, ***P ≤ .005. All data represent the mean plus or minus SEM.