Gfi-1 transduction in 32Dcl3 cells and its effect on CXCR4 expression. (A) GFP expression in 32Dcl3 cells infected with control and Gfi-1 retrovirus was detected by flow cytometry. The profile of noninfected 32Dcl3 cells is also shown (--------). (B) Gfi-1, CXCR4, and GAPDH mRNAs in 32Dcl3 cells infected with control and Gfi-1 retroviruses were detected by semiquantitative RT-PCR. (C) Gfi-1 and actin were detected by Western blotting in cell lysates of 32Dcl3 cells infected with control and Gfi-1 retroviruses; representative results. (D) Western blot analysis of CXCR4 and actin in cell lysates of 32Dcl3 cells infected with control and Gfi-1 retroviruses; representative results. (E) Surface CXCR4 expression in 32Dcl3 cells infected with control and Gfi-1 retroviruses detected by flow cytometry. Background staining with control antibody is shown (--------). (F) Attachment of 32Dcl3 cells infected with control and Gfi-1 retroviruses to wells coated with SDF-1α or medium only. Results are expressed as the percentage of cell input; error bars represent SD of the mean (*P < .05).