AdV induces Stat4- but not Stat1-dependent CD8 T-bet expression in vivo. (A) AdV-GFP gene transfer vectors were injected into footpads of BALB/c mice. On days 1 and 3, CD8 and CD4 T cells were purified from draining LNs, and real-time RT-PCR was used to measure T-bet expression. (B) Real-time RT-PCR for T-bet expression in CD8 and CD4 T cells from Stat1-deficient mice injected with AdV-GFP. (C) Real-time RT-PCR for T-bet expression in CD8 and CD4 T cells from Stat4-deficient mice injected with Adv-GFP. (D) Real-time RT-PCR for IFNγ expression in CD8 T cells from various strains injected with AdV-GFP.