Phenotypic analysis of normal versus leukemic stem-cell populations. Examples of stem-cell phenotyping are shown for GFP control (top left), CML (top right), and bcCML (bottom panels) populations. In each case, cells were first gated on the Lin⁻ population. GFP and CML populations show typical labeling for stem-cell markers c-kit and sca-1. Labeling of bcCML shows reduced c-kit expression (bottom left). The CD150⁻ and Flt3⁺ subpopulation of bcCML cells (bottom right) is highly enriched for LSCs (1 in 7 cells by limiting dilution). Percentages shown in each panel represent the overall frequency in the marrow population.