Identification of Notch1-dependent genes in an E2A^−/− lymphoma. The E2A^−/− lymphoma line 0531 was transduced with the MigR1-ICN retrovirus; 24 hours later, transduced and nontransduced cultures were treated with GSI or DMSO. RNA was extracted 24 hours after addition of GSI or DMSO and used to screen Affymetrix 430A microarrays. The fold change in mRNA expression is shown for nontransduced (■) or MigR1-ICN1 transduced (▩) cells treated with GSI relative to nontransduced cells treated with DMSO. Gene expression was determined by microarray analysis; Nrarp, Notch1, and cyclin D1 were represented by 2 unique identifiers on the microarray.