Figure 4
In vivo regulation of Lef1 by Notch1 in T-cell lymphomas. (A) Schematic of experimental design. (B) Northern blot of mRNA isolated from the spleens of tTA-Nic lymphoma–carrying mice with disease at the indicated time point (hours) after initiation of doxycycline treatment. Two independent experiments are shown. The blots were probed sequentially with cDNA probes specific for Lef1, Tcf1, or actin as indicated. (C) Western blot of protein extracts prepared from splenocytes of diseased mice at the indicated time (hours) after initiation of doxycycline treatment. The blot was probed sequentially with anti-Lef1 and anti-actin antibodies as indicated.
