Examples of CLL-1 expression in AML samples at diagnosis and in normal bone marrow. After labeling of the cells with the appropriate antibody combinations, the CD45^dimCD34⁺CD38⁻ cells were identified by a precise gating strategy (as described in “Fluorescence-activated cell sorting analysis of CD34⁺CD38⁻ cells”; not shown) with subsequent detection of isotype and CLL-1 expression. For the CD34⁻ AML (E-F) only blast gating (CD45^dim) was performed. (A-F) Isotype and CLL-1 expression in AML samples. The percentages in the lower right quadrant indicate CLL-1 expression as a percentage of the CD34⁺CD38⁻ compartment; in the lower left quadrant, the percentage CLL-1⁻ cells within the CD34⁺CD38⁻ compartment is shown. (B) An example of CLL-1 expression on CD34⁺CD38⁻ cells close to the median. (D) An example of a high CLL-1 expression. (F) A representative example of CLL-1 expression in a CD34⁻ AML sample. (H) The absence of CLL-1 expression on CD34⁺CD38⁻ cells of normal bone marrow. Note that CLL-1 is expressed on part of the progenitor population.