Inflammation with poly (I:C) enhances consumption of transfused RBCs by DCs. Syngeneic RBCs were labeled with DiO and transfused into recipient mice that were preinjected intraperitoneally with either poly (I:C) or control PBS. Twenty-four hours after transfusion, spleens and livers were harvested. Macrophages or DCs were visualized by staining with anti-F4/80 or anti-CD11c (not shown). DiO fluorescence was measured after gating on DCs from the spleen (A) or liver (B), or on macrophages from the spleen (C) or liver (D). Cells from mice treated with poly (I:C) are solid lines, whereas control animals are dotted lines. Staining from representative mice is shown, and y-axes of histograms represent percentage of maximum peak value. This experiment was performed 3 times with similar results.