Incorporation of EPC-derived MVs in HMECs. (A) Representative FACS analysis of internalization, after a 30-minute incubation at 37°C, by HMECs of MVs labeled with PKH26 (filled curve). HMECs were incubated with vehicle alone as internal control (open curve). (B-F) Representative FACS analysis of internalization of MVs preincubated (open curves) or not (filled curves) with 1 μg/mL blocking mAb against ICAM-1 (B), with 5 μg/mL soluble Ha (C), 1 μg/mL blocking mAb against α6 integrin (D), α4 integrin (E), CD29 (F). Filled curves indicate the MVs internalized in the absence of blocking Abs or Ha; open curves indicate the MVs internalized after incubation with blocking Abs or soluble Ha. Three experiments were performed with similar results.