Specific reduction of FES, FER, and KIT proteins in cultured cells. FES, FER, and KIT protein expression in TF-1 KIT^D816V cells (A), TF-1 cells (B), and P815 cells (C) treated with the corresponding specific siRNAs were analyzed by Western blot. SCLs were prepared 48 hours following transfection, and 30 μg of proteins were loaded per lane. As loading control, ERK2 expression was assessed. fes A and fes B are 2 independent human fes siRNAs. fes I and fes II are 2 independent mouse fes siRNAs (“siRNA transfection”). (B) — indicates lysate of cells not treated with siRNA. (C) Arrow shows FES protein. *Nonspecific band in P815 cells.