SAHA did not affect translation of cellular proteins in Jeko1 cells. (A) Jeko1 cells were metabolically labeled ([³⁵S]methionine) in either the presence or the absence of SAHA (5 μM). Labeled cells were lysed, and activity of incorporated radioisotope into proteins was measured by scintillation counts. CPM, counts per minute. (B,C) Jeko1 cells were metabolically labeled either in the presence or absence of SAHA (5 μM) for the indicated times. Labeled cells were lysed, and proteins were extracted, fractionated on SDS-PA gel, transferred to membranes, and stained with Ponceau S to determine the amounts of proteins loaded in the lanes (B). Amount of proteins loaded in each lane was well-balanced. (C) The membranes were exposed on X-ray film to determine the levels of incorporated radioisotope in the proteins. Levels of incorporated radioisotopes into proteins increased depending on the duration of labeling, but those levels were not affected by the presence of SAHA.