Figure 5
![Figure 5. Impact of mutations in the C-terminal tyrosines of LAT on PI3K and PLC activation in response to GPVI triggering. [32P]-labeled platelets from WT (♦) and Lat3YF (■) (A) or from WT (Rag−/−) (♦) and LatY136F mice (●) (B) platelets were stimulated by 5 nM Cvx for the indicated times and the levels of [32P]-PtdIns(3,4,5)P3, [32P]-PtdIns(3,4)P2, [32P]-PtdIns(4,5)P2, and [32P]-PtdOH were analyzed as indicated in “Platelet preparation and in vitro aggregation studies.” Results are means (± SEM) of 4 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/110/7/10.1182_blood-2007-02-075432/2/zh80190707710005.jpeg?Expires=1735524892&Signature=siEFgUQXB3Ee9k29GnprBr-AyIzLLTIqUILIK5Ec5FbIqtnnFtsWtFA4pkDpIoY64rg2hMvOB4wBy0mjwZNo0F2-1CtnMMH799x2~w~WVqOtQYnm4V3m2nRUJ6QPjnl0Gx53NLk0UOhDB-qEBFR9jFxPrr1f81IkRRkYk7Q9y6Qspn2KUv5M77PKLGE2IokEd~0NMG2cxP7Z8pdspa9e-IIq-PEAfbfoj53Nx2iTPiwd-mLFRLkX7p~0Zz6MEb3oZlfemuE~M3aJ-ul4uwvRe1uE4l~rqO7BIMbVGht180sOn980PIolKn7-smbZaRLfxBDQMLvYU9fFEV-Ul~uz7A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Impact of mutations in the C-terminal tyrosines of LAT on PI3K and PLC activation in response to GPVI triggering. [32P]-labeled platelets from WT (♦) and Lat3YF (■) (A) or from WT (Rag−/−) (♦) and LatY136F mice (●) (B) platelets were stimulated by 5 nM Cvx for the indicated times and the levels of [32P]-PtdIns(3,4,5)P3, [32P]-PtdIns(3,4)P2, [32P]-PtdIns(4,5)P2, and [32P]-PtdOH were analyzed as indicated in “Platelet preparation and in vitro aggregation studies.” Results are means (± SEM) of 4 experiments.
