Integrin expression in mouse fetal liver HSCs. E14.5 FL cells were stained with lineage antibodies (Lin) and gated for Lin⁻ and viable cells. Lin⁻ viable cells were gated for Sca-1⁻c-Kit⁺ clonogenic progenitor cells and the more primitive Sca-1⁺c-Kit⁺ (LSK) cells. The left panels show gates for Sca-1⁺c-Kit⁺ and Sca-1⁻c-Kit⁺ on Lin⁻ cells. A ubiquitous expression of integrin α6, α4, and α5 chains was seen in LSK cells (right panels) and Lin⁻Sca-1⁻c-Kit⁺ progenitors (middle panels). Staining with the anti-integrin antibodies is shown as open histograms and staining with isotype control antibodies, as gray histograms. Shown is 1 representative analysis of 2.