American Society of Hematology

Figure 5

$Figure 5. Perturbation of fetal liver HSC homing by antibodies against integrin α6 and α4. (A) Schematic representation of the experimental setup: FL cells from CD45.2 mice were incubated with rat IgG2a antibody (rIgG) or antibodies against integrin α4 (α4) or integrin α6 (α6) and injected into lethally irradiated CD45.1 mice. BM cells were harvested 3 hours after injection and cells from one femur each were injected together with CD45.2 competitor BM cells into lethally irradiated CD45.1 secondary recipient mice. Blood was harvested at 3, 6, and 16 weeks after transplantation and reconstitution was analyzed by flow cytometry. (B) Peripheral blood reconstitution of CD45.2 cells and CD45.2 myeloid cells at 3, 6, and 16 weeks. ***P < .001 compared with reconstitution from cells incubated with rat IgG. Data are pooled from 2 independent experiments. The horizontal bars show mean values. (C) Representative FACS dot plots showing blood reconstitution 16 weeks after secondary transplantation. The left panels show reconstitution of CD45.2 FL cells. The right panels show blood reconstitution of myeloid (Mac-1) cells derived from CD45.2 cells. The numbers indicate the mean percentage of cells in the corresponding fraction (n = 11-14).$

Perturbation of fetal liver HSC homing by antibodies against integrin α6 and α4. (A) Schematic representation of the experimental setup: FL cells from CD45.2 mice were incubated with rat IgG2a antibody (rIgG) or antibodies against integrin α4 (α4) or integrin α6 (α6) and injected into lethally irradiated CD45.1 mice. BM cells were harvested 3 hours after injection and cells from one femur each were injected together with CD45.2 competitor BM cells into lethally irradiated CD45.1 secondary recipient mice. Blood was harvested at 3, 6, and 16 weeks after transplantation and reconstitution was analyzed by flow cytometry. (B) Peripheral blood reconstitution of CD45.2 cells and CD45.2 myeloid cells at 3, 6, and 16 weeks. ***P < .001 compared with reconstitution from cells incubated with rat IgG. Data are pooled from 2 independent experiments. The horizontal bars show mean values. (C) Representative FACS dot plots showing blood reconstitution 16 weeks after secondary transplantation. The left panels show reconstitution of CD45.2 FL cells. The right panels show blood reconstitution of myeloid (Mac-1) cells derived from CD45.2 cells. The numbers indicate the mean percentage of cells in the corresponding fraction (n = 11-14).

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