Influence of RNase pretreatment on venous thrombosis in rats. Vessel occlusion was induced by FeCl3 exposure to the superior sagittalis sinus. At 30 minutes prior to the start of the experiment, animals were pretreated with either saline (Control), RNase, DNase, or heparin, respectively. At different time points after induction of thrombosis, (A) vessel occlusion rate and (B) bleeding times were determined. Values are expressed as the mean (± SEM; n = 12 for each animal group). (C) Hematoxylin-eosin staining of paraffin-embedded brain sections prepared from saline-treated (control), RNase-treated, or heparin-treated rats, respectively, was performed. Note the occluded vessel (✶) and massive perivascular edema formation (→) in the control, whereas in the RNase treatment group, greatly reduced vessel occlusion (✶) and hardly any edema were seen. In the heparin treatment group, no vessel occlusion (✶) is seen, whereas edema formation (→) remained. Slides were inspected with a DMRB Leica microscope, magnification was 40×/1.00-0.5 NA objective. Images were taken using a digital camera DFC300FX and image-acquisition software IM500 form Leica.
