GSE24-2 increases hTERT promoter activity. (A) 293T cells were cotransfected with pLNCX, DKC, GSE24-2, Cbf5, motif I, or motif II (10 μg DNA per million cells) and the hTERT-luc reporter (1 μg per million cells). Luciferase activity was measured following 24 hours of transfection. Asterisk indicates values with statistical significance of P <.05. (B) 293T (■) and 293T24-2 (□) cells were cotransfected with the hTERT-luc reporter (1 μg per million cells). After 24 hours, the cells were treated with cisplatin (3 μg/mL) for 8 hours and the luciferase activity was determined. (C) 293T cells were cotransfected with the indicated constructs (10 μg DNA per million cells) and the hTR-luc reporter (1 μg per million cells). Luciferase activity was measured following 24 hours of transfection. (D) Cell lines were cotransfected with the hTERT-luc reporter and different amounts of the Mad/myc expression vector. Luciferase activity was measured following 24 hours of transfection. (E) Cell lines were cotransfected with the HIV-luc reporter and increasing amounts of the mad/myc expression vector. Following 24 hours of transfection, cells were stimulated with 50 ng/mL TNFα for 6 hours and luciferase activity was measured. CMV-Renilla (0.1 μg/mL per million cells) was used as a control for transfection efficiency. Data points represent the mean and standard deviations of 2 experiments performed in quadruplicate.