Comparison of PAX2 protein and mRNA expression in HMEC-S and in HMEC/HHV-8. (A) PAX2 mRNA expression analyzed by quantitative real-time PCR was performed for using RNA extracted from HMEC-S stably transfected with sense vector of PAX2 and HMECs infected with HHV-8. The normalized expression of PAX2 gene with respect to beta2 microglobulin was computed for all samples. Values are fold changes with respect to control and are the mean plus or minus SD of 2 independent experiments performed in triplicate. Mann-Whitney nonparametric test was performed (* P < .05, HMEC-S and HMECs/HHV-8 vs control HMECs). (B) Representative Western blot analysis of PAX2 protein expression by control HMECs, HMEC-S, and HMECs 3 days after HHV-8 infection showing a band corresponding to 46 kDa.