COFRADIC N-terminal analysis revealed preferential down-regulation of proteins related to protein translation/RNA-binding in DNR/CHX-treated NB4 cells. (A) Gene Ontology-based analysis of the COFRADIC protein data according to biologic function. The inner ring (a) represents the biologic process relative values of all the proteins identified in the analysis. The middle ring (b) represents the N-terminal down-regulated proteins, and the outer ring (c) represents the protein substrates producing stable cleavage products. The pie chart visualizes a striking preferential down-regulation of proteins involved in translation (b). The chart was generated using the PIGOK analysis; http://128.40.158.133/pigok.html. (B) HL60 cells were treated with 1.6 or 8 μmol/L DNR for the periods indicated, and extracts were immunoblotted and probed for eEF2K and eEF2.