Added LTB4 induced SAP expression and inhibition of B-cell proliferation in the EBV-infected cord blood cell cultures. (A) SAP expression. Immunoblot as in Figure 1A. EBV-infected CBMCs were cultured for 6 days with LTB4, LTC4, LTD4, or 5(S),12(S)-DiHETE at 100 nM concentration. The results show 1 of 3 experiments. (B) Dose response of the added LTB4; 1 representative experiment of 4 performed. (C) Cell composition in EBV-infected CBMCs cultured with LTB4 for 6 days. The numbers denote the percentage of CD3+, CD56+, and CD19+ cells. (D) EBV-induced B-cell proliferation in the LTB4-containing cultures. 3H-thymidine incorporation was measured on the 6th day of culture, 3H-thymidine was present during the final 14 hours. The cultures were initiated with 2 × 105 cells. The results represent the means plus or minus SD of 4 independent experiments. * indicates P < .05, and **P < .01 compared with untreated cultures. (E) Isolated B cells were infected with EBV and cultured with LTB4. The cultures were initiated with 105 cells. Thymidine incorporation was measured on the 6th day of culture. The results represent the means plus or minus SD of 4 independent experiments.
