Contribution of monocytes (Mo's) and T lymphocytes to the SAP expression induced by LTB4 in the EBV-infected cultures. SAP expression was tested on the 6th day in all cultures. (A) EBV-infected CBMCs, total, and monocyte (Mo)–depleted populations were cultured with 1 nM, 10 nM, and 100 nM LTB4. One representative experiment of 4 performed. (B) B-cell proliferation. Total and monocyte (Mo)–depleted populations infected with EBV, and cultured without or with 100 nM LTB4. 3H-thymidine incorporation was measured on the 6th day of culture. The results represent the means plus or minus SD of 3 independent experiments. ** indicates P < .01 compared with untreated cultures. (C-E) Monocyte-depleted populations were infected with EBV. The separated monocytes were exposed to LTB4 for 20 hours, and thereafter reintroduced to the depleted population. The cultures were kept for further 5 days. (C) SAP expression. The separated monocytes were treated with LTB4 (100 nM), PSK (25 μg/mL), and Trx80 (100 nM). LTB4 was added to the cultures as indicated. The results show 1 representative of 3 experiments. (D) LTB4 (10 nM or 100 nM)–treated monocytes were reintroduced to the cultures. MK886 (1 μM) and BWA4C (100 nM) were added as indicated. (E) LTB4 (100 nM)–treated monocytes were reintroduced to the cultures. Anti–IL-18 (2 μg/mL), anti–IL-15 (10 μg/mL), and anti–IL-12 (10 μg/mL) reagents were added as indicated. (F) Cell proliferation. EBV-infected CBMC cultures without and with LTB4. To parallel samples, antibodies anti–IL-18 (2 μg/mL), anti–IL-15 (10 μg/mL), and anti–IL-12 (10 μg/mL) were added as indicated. 3H-thymidine incorporation was measured on the 6th day of culture. The results represent the means plus or minus SD of 3 independent experiments. * indicates P < .05, and **P < .01 compared with untreated EBV-infected cultures. (G) SAP expression. EBV-infected CBMCs, total, and monocyte (Mo)–depleted populations were cultured with 10 ng/mL IL-18. The results show 1 representative of 3 experiments. (H) SAP expression in monocyte and T-cell–depleted cultures. (i) Monocyte depleted cultures were infected with EBV and cultured without or with LTB4 (100 nM). On the sixth day of culture the cells were collected and the T cells were depleted before preparation of the lysates, which were then tested for SAP expression. (ii) Cell population depleted of Mo and T cells were infected with EBV and cultured without or with LTB4. The results show 1 representative of 3 experiments.
