miRNA expression pattern in cHL. (A) Unsupervised hierarchic cluster analysis categorized 3 clusters corresponding to nodular sclerosis (NS), mixed cellularity (MC), and reactive lymph nodes (RLNs). A corresponding typical histology is shown. The data were presented as log₁₀ of relative quantification normalized in regard to global median and relative to the 10 reactive lymph node median as calibration method. (B) A set of 55 miRNAs classified all samples into NS, MC, or RLN (PAM analysis). In blue, the 25 miRNAs comprising the miRNA expression signature capable of distinguishing between cHLs and RLNs.