CD4⁺ and CD8⁺ T-cell proliferation in cultures with autologous DCs pulsed with TT. Immature DCs pulsed with TT or unpulsed (−), were subjected to differentiation in the presence of TNF-α and subsequently irradiated prior to coculture, as in the case of mIg. The proliferative response of T cells from 6 patients with MM (nos. 1, 2, 5-8) and 4 healthy blood donors (Ctrls), all of whom had been vaccinated against TT, were analyzed after 7 days of coculture with TT-pulsed DCs. The mean percentages plus SD (left) and total numbers/culture plus SD (right) of dividing “CFSE-low” CD4⁺ and CD8⁺ T cells are depicted. There was no significantly different T-cell response towards TT between the patients with MM and control subjects. Significant differences comparing the CD4⁺ and CD8⁺ T-cell proliferation in cultures with TT-pulsed versus unpulsed DCs are indicated (**P < .01; *P < .05; t test).