Anti-μ–induced increases in phosphorylated CD79b in ZAP-70+ versus ZAP-70− CLL cells or ZAP-70− CLL B cells made to express ZAP-70-Wt or ZAP-70-KA369. (A) Each bar represents the mean percentage increase in phosphorylated CD79b observed following treatment with anti-μ of CLL B cells that were ZAP-70+ (N = 28) or ZAP-70− (N = 19), as indicated at the bottom of the figure. The error bars indicate the standard deviation of the mean. The difference in the mean percentage increase in ZAP-70+ CLL cells versus ZAP-70− CLL cells was significant, as indicated by the P value at the top of the figure. (B,C) Anti-μ–induced increases in the level of phosphorylated CD79b detected in ZAP-70− CLL cells transduced with a control adenovirus or Ad-ZAP-70 (B), or control adenovirus or Ad-ZAP-70-KA369 (C). The lines connect the percentage increase in detected phosphorylated CD79b of anti-μ–treated CLL cell samples transduced with Ad-lacZ (“Control” in each panel) versus that measured in the same CLL cells that had been transduced with Ad-ZAP-70 (B) or Ad-ZAP-70-KA369 (C).
