Strong induction of LPAM-1, but less induction of E-lig and P-lig by MLN DCs. (A) Four days after coculture with different organ-derived dendritic cells, expression of LPAM-1, LFA-1, E-lig, P-lig, integrin α₄, integrin β₁, and integrin β₇ allogeneic T cells was analyzed. Effector memory cells (CD44^hiCD62L^lo) in the MLN DC group express LPAM-1 and significantly less E-lig or P-lig (*P < .001, n = 8-15). There is also statistically significantly higher expression of integrin α₄ and integrin β₇ in the MLN DC group (**P < .05, n = 5-6). Error bars represent SEM. (B) Coculture of PLN and MLN DCs at equivalent concentrations shows that MLN DCs induce LPAM-1 irrespectively of PLN DCs, and negatively affect the induction of E-lig and P-lig (1 representative of 3 independent experiments).