American Society of Hematology

Figure 1

$Figure 1. Surface BCR expression on human IgM and IgA PCs. (A) In vitro differentiated memory cells were stained for CD138 and membrane (mb) and intracellular (ic) Ig; 1 representative experiment of 4. (B) Expression of ic and mb IgG, IgA, and IgM in CD19+CD138+-gated BM-PCs; 1 representative experiment of 3. (C) Expression of CD20 and CD138 on the CD38-enriched fraction of colon LP (left panel). Expression of mb and ic IgA in CD3–/CD38high LP-PCs (right panel); 1 experiment of 2. (D) Igα and Igβ mRNA in BM-PCs and memory B cells normalized to 18S RNA. Mean + standard error of the mean (SEM) of 3 donors. (E) Expression of intracellular epitopes (gray histograms) of Igα and Igβ on IgG, IgA, and IgM BM-PCs. Control staining, open histograms; 1 experiment of 3. (F) Expression of Blimp-1 and Pax-5 on BM-PCs and memory B cells. Mean of rMFI + SEM of duplicates; 1 experiment of 3.$

Surface BCR expression on human IgM and IgA PCs. (A) In vitro differentiated memory cells were stained for CD138 and membrane (mb) and intracellular (ic) Ig; 1 representative experiment of 4. (B) Expression of ic and mb IgG, IgA, and IgM in CD19⁺CD138⁺-gated BM-PCs; 1 representative experiment of 3. (C) Expression of CD20 and CD138 on the CD38-enriched fraction of colon LP (left panel). Expression of mb and ic IgA in CD3^–/CD38^high LP-PCs (right panel); 1 experiment of 2. (D) Igα and Igβ mRNA in BM-PCs and memory B cells normalized to 18S RNA. Mean + standard error of the mean (SEM) of 3 donors. (E) Expression of intracellular epitopes (gray histograms) of Igα and Igβ on IgG, IgA, and IgM BM-PCs. Control staining, open histograms; 1 experiment of 3. (F) Expression of Blimp-1 and Pax-5 on BM-PCs and memory B cells. Mean of rMFI + SEM of duplicates; 1 experiment of 3.

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