Host CD8⁺DCs are dispensable for induction of allo-reactivity and GVHD severity. (A) Survival (n = 6 to 17 per group, pooled from 2 experiments) and (B) clinical GVHD score after allo-HCT (n = 6 to 17 per group, pooled from 2 experiments). (C) Donor CD45.2⁺CD8⁺ T cells (syngeneic) or CD229.1⁺CD8⁺ T cells (allogeneic) (n = 8 to 12 per group, pooled from 3 experiments) expansion in spleen on day 14. (D) The subpopulation of donor-type CD8⁺ T cells. Effector memory (EM; CD62L⁻CD44⁺), central memory (CM; CD62L⁺CD44⁺) and naive (CD62L⁺CD44⁻) CD8⁺ T cells were based on the expression of CD62L and CD44 (n = 6 to 8 per group, pooled from 2 experiments). (E) Donor-type IFN-γ⁺ and (F) granzyme B⁺ CD8⁺ T cells (n = 8 to 17 per group, pooled from 3 experiments) in spleen on day 14. (G) Donor-type CD4⁺CD25⁺Foxp3⁺ regulatory T cells on day 14 (n = 8 to 12 per group, pooled from 3 experiments). Serum levels of (H) IFN-γ and (I) IL-17A on day 14 (n = 8 to12 per group, pooled from 3 experiments). (C-I) The bars represent mean ± standard deviation. N.S. denotes no significant differences (P > .05).